Abstract Purpose: To evaluate the morphological effects of injected sclerosing agents into the liver. Methods: This study was performed on twenty dogs, distributed into five groups: Group 1 (n = 5) - control, Group 2 (n = 5) - injection of 50% glucose solution inside hepatic parenchyma and animals followed during seven days, Group 3 (n = 10) - injection of ethanol inside hepatic parenchyma and animals distribution into two subgroups Subgroup 3A (n = 5) - followed during 24 hours and subgroup 3B (n = 5) - followed during seven days (group 3B), Group 4 (n = 5) - ethanol injection inside left portal vein branch and followed during 24 hours. Livers were macroscopically evaluated, submitted to hepatic arteriography and portography, then histology. Results: All animals in Group 4 died within 23 hours due to diffuse hepatic necrosis. The animals of groups 2 and 3 had a satisfactory evolution. Fibrosis formed in the segment reached by the sclerosant solution and interruption of the contrast flow injected into the portal system. Conclusion: Intrahepatic parenchymal ethanol injection is well tolerated and causes sclerosis restricted to a specific segment; however, intraportal ethanol injection causes massive hepatic necrosis and can lead to death.

A ligadura da veia renal esquerda é uma alternativa operatória em presença de lesão deste vaso. O objetivo deste estudo foi avaliar a capacidade dos afluentes dessa veia, proximais à ligadura, de manter a vitalidade tecidual e a função renal. Foram estudados 15 cães mestiços, machos, divididos em três grupos de cinco cães. No Grupo I (Controle), foi realizada apenas laparotomia e identificação das estruturas. Os animais do Grupo II foram submetidos a laparotomia mediana seguida de ligadura da veia renal esquerda, próximo à veia cava. Nos animais do Grupo III, foi realizada a ligadura da veia renal esquerda, próximo à veia cava e nefrectomia direita. Determinaram-se as concentrações plasmáticas de uréia e creatinina, em todos os animais, no período pré-operatório e durante as quatro semanas pós-operatórias, a cada três dias. Nos animais que sobreviveram até o final do período de acompanhamento, realizaram-se arteriografia renal e urografia excretora. Após este período ou depois de óbito precoce, os rins foram retirados para estudo histológico. Todos os animais do Grupo III morreram antes de dois meses de seguimento (M=10,5 ± 3,2 dias), enquanto os animais do Grupo II sobreviveram durante este período. Houve exclusão completa do rim esquerdo em todos os animais submetidos à ligadura venosa. O exame anatomopatológico mostrou, no Grupo II, desaparecimento do limite corticomedular e, microscopicamente, quadro sugestivo de nefrosclerose. No Grupo III, constatou-se aumento do rim esquerdo e grande quantidade de coágulos intravasculares, intrapélvicos e subcapsulares. Microscopicamente, observaram-se extensas áreas de necrose de coagulação, infiltrado inflamatório e hemorragia. Concluindo, as tributárias da veia renal esquerda foram insuficientes para manter a vitalidade tecidual e a função renal, após a ligadura de sua veia principal.

The ligature of the left renal vein is an alternative whenever this vessel is injured. The purpose of this study was to evaluate the capacity of the affluents of the left renal vein, proximal to the ligature, to maintain tissue vitality and function of the left kidney. Fifteen mongrel male dogs were divided in 3 groups of 5 dogs: Group I (control) - a laparotomy was performed, and the abdominal structures were only identified; Group II - the left renal vein was tied, close to vena cava; Group III - the same procedure as for Group II and a right nephrectomy. Blood urea nitrogen and serum creatinine levels were measured before the procedure, and every 3 days during 4 weeks in the postoperative period. Renal arteriography and an excretory urogram were performed on the animals that survived 60 days. Thereafter, or immediately after precocious death, the kidneys were removed for histological examination. All the animals of Group III died before two months (mean = 10.5 +-3.2 days), while the animals of Group II survived during that period. There was a complete exclusion of the left kidney in all dogs that underwent renal vein ligature. In the animals of Group II, the renal cortico-medullary limits could not be identified. At microscopy, the aspect was suggestive of nephrosclerosis. In the animals of Group III, the left kidney was enlarged, and a great amount of intravascular and intrapelvic blood clots were observed. At microscopy, extensive areas of necrosis, inflammatory infiltration, and hemorrhage were identified. In conclusion, the tributaries of the renal vein were not sufficient to maintain the tissue vitality and function of the left kidney after ligature of its main vein.